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KMID : 0545120070170060934
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 6 p.934 ~ p.944
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Proteome Analysis of Paenibacillus polymyxa E681 Affected by Barley
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Seul Keyung-Jo
Park Seung-Hwan
Ryu Choong-Min
Lee Yong-Hyun
Ghim Sa-Youl
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Abstract
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Paenibacillus polymyxa E681 is known to be able to suppress plant diseases by producing antimicrobial compounds and to promote plant growth by producing phytohormones, and secreting diverse degrading enzymes. In spite of these capabilities, little is known regarding the flow of information from the bacterial strain to the barley roots. In an attempt to determine the flow of information from the bacterial strain to barley roots, the strain was grown in the presence and absence of barley, and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and MALDITOF mass spectrometry were used. 2D-PAGE detected approximately 1,000 spots in the cell and 1,100 spots in the supernatant at a pH 4-10 gradient. Interestingly, about 80 spots from each sample showed quantitative variations. Fiftythree spots from these were analyzed by MALDI-TOF mass spectrometry and 29 proteins were identified. Most of the cytosolic proteins expressed at higher levels were found in P. polymyxa E681 cells grown in the presence of barley rather than in the absence of barley. Proteins detected at a lower level in the surpernatant of P. polymyxa E681 cells grown in the presence of barley were lipoprotein, glucose-6-phosphate 1-dehydrogenase, heat-shock protein HtpG, spermidine synthase, OrfZ, ribonuclease PH, and coenzyme PQQ synthesis protein, whereas proteins detected at a higher level in the surpernatant of P. polymyxa E681 cells grown in the presence of barley included D-alanyl- D-alanine ligase A, isopentenyl-diphosphate delta-isomerase, ABC transporter ATP-binding protein Uup, lipase, and flagellar hook-associated protein 2. Many of the proteins belonging to plantinduced stimulons are associated with biosynthetic metabolism and metabolites of proteins and transport. Some of these proteins would be expected to be induced by environmental changes resulting from the accumulation of plant-secreted substances.
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KEYWORD
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Paenibacillus polymyxa E681, barley, bacteriaplant interaction, 2D PAGE, proteome
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